不同保存方法对光皮桦总DNA提取效果的影响

光皮桦是一种顽拗类植物,其鲜叶组织中富含酚类、糖类及其他次生代谢物。这些物质很容易使鲜叶发生褐变,从而严重影响植物基因组DNA的提取质量。本文以新鲜嫩叶为对照,比较了-20℃冷藏、硅胶干燥、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液固定4种鲜叶保存方法对提取光皮桦基因组总DNA效果的影响,寻找最佳的光皮桦嫩叶保存方法。并以提取的核酸得率、纯度、片段分布情况、是否含有PCR反应抑制剂等指标来评价其保存效果。结果表明:-20℃冷藏的样品未能提到DNA;新鲜样品、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液保存的样品均提到纯度较好的DNA,大小在48Kb左右,得率为400!g/g鲜叶左右,并获得条带清晰、多态性好的PCR扩增图谱;用硅胶干燥保存的样品也能提到DNA,但得率低,为51.2!g/g鲜叶,且未获得条带完整、清晰的PCR扩增图谱。     

Some Ideas on Reconstruction of Villages inside the City

In this paper, the historical reasons of formation of the village inside the city are analyzed. It is indicated that such situation for the villages inside the city are the result of joint action of both the villagers and the government. The foundation of villages inside the city laid on the simple compensation for farmers in confiscating the rural land and lack of intelligence aids. In this paper, the ideas and measures for their reconstruction are put forward based on consideration of the reasons of their formation and difficulties in the reconstruction.     

AN APPRONTMATE CALCULATING METHOD FOR WALL PLATES OF MAN'' S DRY GAS HOLDERS

The basic equations of wall plates of MAN' S dry gas holders are derived in this paper. According to the law of cylindrical bending given by the finite element results .we derive some approximate formula calculating the deflection and stresses of the wall plates from the basic equations. Compared with the known approximate calculating method.the method car. obviously increase the percision.     

Ecological Technology Strategy-the Typologic Analysis and Research of Double-Skin Facade

As a renovation envelope, double-skin facade has the characteristics of environmental function and unique aesthetic effect. In this paper, the composition and functional characteristics of double skin facade is introduced briefly, its development history and a detailed typologic analysis combined with typical cases is reviewed. It is pointed out that the double skin facade has a bright future in 21~(st) century and the function and aesthetics are the footstone for its typologic research.     

Structural Analysis and Discussion of Wide -stayed Bridge Basket

Based on a cable -stayed bridge girder as an example, the structure and function, the operating principle and calculation model of the main elements of the Basket of wide - stayed bridge were described in detail. A further discussion was carried out on the Basket of Cantilever construction of the main beam in the structure.     

三个不同品质类型冬小麦品种籽粒淀粉积累动态及其有关酶的活性变化

在池栽条件下，研究了3个不同品质类型冬小麦品种藁麦8901（强筋）、豫麦49（中筋）和洛麦1号（弱筋）籽粒灌浆过程中淀粉及其组分积累动态和与之有关的酶活性变化。结果表明，3个品种籽粒灌浆过程中支链淀粉的合成均与直链淀粉的合成同时进行，灌浆中后期支链淀粉的合成比直链淀粉的合成快。豫麦49籽粒中直链、支链和总淀粉积累速率均高于其它两品种。3个品种籽粒中腺苷二磷酸葡萄糖焦磷酸化酶（AGPP）、尿苷二磷酸葡萄糖焦磷酸化酶（UGPP）、可溶性淀粉合成酶（SSS）、淀粉粒结合的淀粉合成酶（GBSS）、淀粉分支酶（SBE）活性变化均呈单峰曲线。豫麦49在灌浆期上述各酶活性变化的峰值均高于其他两品种，且大部分酶类在灌浆中后期仍维持较高活性。可见，与藁麦8901和洛麦1号相比，豫麦49具有较强的淀粉合成能力。     

江淮稻区不同穗型粳稻品种主要农艺和品质特性的比较分析

依据着粒密度，对江淮稻区大面积生产应用的和新近育成的36个粳稻品种(系)进行聚类分析。结果表明，供试品种可分成半密穗型(Ⅰ)、偏密穗型(Ⅱ)和密穗型（Ⅲ）三类。随着水稻品种由散穗型向半密穗型、偏密穗型和密穗型的演变，产量性状、品质性状、形态性状和部分生理、生化性状发生了明显的变化，产量、每穗总粒数和实粒数显著增加，而穗数/m²、千粒重和结实率呈下降趋势；株高增加，上部三张叶片变长、变宽，生物日产量增高；穗部一、二次枝梗数增多，着粒密度变大；抽穗期和成熟期叶片、茎秆的含氮量和叶片叶绿素含量增高；糙米率和整精米率下降、垩白粒率降低、胶稠度变硬、蛋白质含量呈上升的趋势。     

The Method of Diagnosing Brain-dysfunction Based on Membership Function and BP Neural Network

Membership functions.formed with the characteristic values obtained by applying the continuous reaction time (CRT) theory from controls and heptic encephalopthy and proposedin this paper.whith these membership functions,the CRT data to be diagnosed are calculated in advance,and then discriminated by BP meural network to differentiate patients with or without braindysfunction.The troubles of low accuracy and efficiency,encountered in training BP network withfuzzy samples.scattered data and extended samples,are solved.